Biobanking human tissues for both nPOD and CARE-T1D programs including receipt of tissues, gross and fine dissection, tissue fixation, freezing, inventory, and distributions. Vibratome cutting of live tissue slices, development and implementation of perifusion protocols to assess glucose-stimulated insulin secretion. Next-day shipment coordination with nPOD research investigators. Establishing new and revised tissue processing protocols for current laboratory practices as well as new projects. On-call rotation schedule for both nPOD and CARE-T1D case processing (nights and weekends).
Original research publications, reports, and abstract writing. Research method development including study design, data collection, immunostaining, CODEX, image analysis, statistical calculations, and background literature review. Reporting program progress and utilization at nPOD and CaRe-T1D meetings. Create figures, graphs, charts, and graphical illustrations for publications, poster presentations, and reports using Microsoft Excel, Powerpoint, Adobe Illustrator, and GraphPad Prism.
Supervise and train lab personnel to ensure adherence to SOPs in day-to-day activities. Evaluating new equipment for purchase. Training lab staff on new equipment and SOPs. Routine maintenance of equipment and coordination with 3rd party vendors to diagnose and troubleshoot equipment failure.
Perform micro CT X-ray microscope operation and data analysis. Provide 3D renderings using 3D.Suite software and calcification volume for online data sharing with consortium members.
Assist in routine immunostaining, frozen tissue sectioning using cryostat, and QC Aperio online pathology system to ensure core histology screening is up to date. Assist in frozen tissue section cutting for investigator requests.
Developing new lab procedures as requested by nPOD investigators and approved by nPOD Tissue Prioritization Committee. Recommending and implementing operating policies and procedures based on international biorepository standards. SOP creation.
Assist the lab managers with other tasks as needed
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