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This is an intermediate level Biological Scientist II position in a research lab studying extracellular matrix/proteoglycans in a wide range of diseases including lung injury and development, pulmonary and vascular biology, pulmonary surfactants, innate immunity, infections, and cancer. The Biological Scientist is expected to be able to 1. Maintain a necessary rodent colony to achieve research objectives; 2. Conduct rodent procedures as necessary to complete research objectives; 3. Oversee their execution assigned experiments; 4. Assist in the design laboratory experiments; 5. Assist in developing/editing protocols to improve existing techniques or to incorporate new diagnostic procedures; 6. Analyze/interpret scientific results; 7. Present researching findings to PI, at laboratory meetings and at scientific meetings; 8. Responsible for the management of laboratory functions; 9. Maintain laboratory safety programs and train personnel in laboratory safety techniques; and 10. Contribute to the writing of grants, papers, and statements of work
The Biological Scientist II must be able to perform the following essential functions:
Animal Colony Management:
Responsible for the management of all rodent lines to achieve research objects. Develop, organize, and conduct experimental studies with live specimens. Handling mice for setting up timed matings, weaning animals and maintaining colonies. Participating in all rodent procedures. Ensuring compliance with IACUC and NIH standards.
Animal Care:
Responsible for the day-to-day management of the rodent colony following all applicable rules and regulations. Identifies incoming animals and houses according to specific requirements; maintains identification of animals using cage cards and tags. Observes animals; recognizes and report early manifestations of disease such as labored breathing, lethargy, sores, and dehydration. Observes and reports animals for signs of illness, injury, and physical and behavioral changes. Follows established procedures to minimize contamination and insure optimum results for researchers. Monitors health status of postsurgical and experimental animals
Histology, Biomedical and Functional Studies:
Molecular cloning, Plasmid purification, Primer design, Protein analysis, RNA and DNA isolation, isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines. Participate in in-vitro studies. Collecting organs and other tissue and embedding the specimens for sectioning either with a microtome or cryotome (for frozen sections). Following preparation of slides with sectioned tissue, staining with standard histology stains such as H&E, as well as with immunohistochemistry/immunofluorescence. Participating in the image capture and analysis of tissue following intervention studies.
Laboratory Management:
Ensuring compliance with all regulatory bodies (IACUC, IRB, EH&S, Etc). Categorizing and storing sensitive scientific materials/instruments. Assisting administration in the reconciling of charges in accordance with cost accounting standards. Orders supplies for the research laboratory.
Other:
Perform tasks and experiments as directed by the Principal Investigator.
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