Job Description: |
Our laboratory is recruiting a postdoctoral fellow to use unbiased approaches (RNA-Seq, scRNA-Seq, spatial profiling, and flow cytometry) to identify novel molecular and cellular events in solid cancers with different treatments. Functional validation of results using standard molecular biology techniques will be performed in vitro and in vivo using patient derived xenograft line, primary samples and murine models. The ideal applicants should have a doctoral degree (PhD or MD/PhD) with ideally (not mandatory) background in cancer biology, tumor immunology, and immunotherapy with experience in:
- Molecular biology for cell genetic engineering of immune cells (e.g., CAR T cell production) and tumor cells using techniques such as CRISPR, viral transduction, cloning, subcloning and plasmid transfection (e.g., PiggyBac)
- Bioinformatics (bulk and single cell RNA sequencing)
Preference will be given to candidates with qualifications also in:
- Molecular Biology (e.g., RNA extraction/production/sequencing, WB, PCR)
- Cloning, subcloning
- Cell culture (tumor cells and immune cells such as dendritic cells and T cells)
- Immunohistochemistry
- Microscopy
- Flow cytometry
- Animal surgery and monitoring
Duties:
Design and develop research projects with the Principal Investigator
Lead at least two independent research projects simultaneously alongside collaborative projects with other team members
Conduct experiments using a variety of techniques including qPCR, RT-qPCR, chromatin immunoprecipitation, cloning/subcloning, immunoprecipitation, immunofluorescence, Western blotting, FACS, mass spectrometry, transcriptomics, CRISPR-Cas9, etc.
Perform data collection and analysis
Initiate and maintain cell cultures
Prepare and maintain biological stocks
Maintain proper lab and experimental records, both digital and hard copy
Test, optimize, troubleshoot new and existing methods and techniques, and update protocols
Guide and train undergraduate and graduate student researchers
Prepare scientific results for publication in peer-reviewed journals and grant applications
Maintain laboratory equipment and order supplies as needed
Present results at internal and external meetings and scientific conferences
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Preferred Qualifications: |
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Special Instructions to Applicants: |
Applications should include a curriculum vitae (CV), and a cover letter, and three letters of recommendations or contact information for three professional references.
Review of applications will begin on immediately and will continue until a sufficient applicant pool has been established.
Final candidate will be required to provide official transcript to the hiring department upon hire. A transcript will not be considered “official” if a designation of “Issued to Student” is visible. Degrees earned from an education institution outside of the United States are required to be evaluated by a professional credentialing service provider approved by National Association of Credential Evaluation Services (NACES), which can be found at
If an accommodation due to a disability is needed to apply for this position, please call 352-392-2477 or the Florida Relay System at 800-955-8771 (TDD). Hiring is contingent upon eligibility to work in the US. Searches are conducted in accordance with Florida's Sunshine Law.
The University of Florida is committed to non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, gender identity and expression, marital status, national origin, political opinions or affiliations, genetic information, and veteran status in all aspects of employment including recruitment, hiring, promotions, transfers, discipline, terminations, wage and salary administration, benefits, and training.
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