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The selected candidate will be primarily responsible for performing in vitro and in vivo experiments using molecular, biochemical and cellular assays in cell culture systems and animal models; Defining goals for research projects and experiments in conjunction with the Principal Investigator (PI) and other research staff; and maintaining and managing all laboratory animal colonies. The candidate will work under the direct supervision of the PI.
The specific duties include, but are not limited to:
• Perform subretinal injections to deliver AAV viral vectors to animal models and evaluate retinal structure and function in laboratory animals using noninvasive vivo imaging technologies (OCT, ERG, fundoscopy).
• Evaluate data by performing appropriate mathematical or statistical calculations and analysis. Compile data using Photoshop, Excel, GraphPad, ImageJ, and PowerPoint.
• Dissect and collect mouse ocular tissues and perform histology and Western Blot analysis.
• Use microscopy to examine and photograph specimens.
• Maintain accurate electronic laboratory databases, records, procedures, and results.
• Verify that equipment and instrumentation are kept in working condition to ensure accuracy and quality of experimental results.
• Participate in laboratory safety programs to ensure compliance with federal regulations and internal regulatory bodies such as IACUC and EH&S.
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| Preferred Qualifications: |
Candidate should have significant work experience with subretinal/intravitreal injection procedures in laboratory animals (includes knowledge of AAV gene delivery systems, mouse anesthesia and recovery, intraperitoneal drug administration), immunostaining and Western blotting, strong organizational skills, and maturity.
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| Special Instructions to Applicants: |
In order to be considered, you must upload a cover letter, resume, and list of references.
The University of Florida is committed to non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, gender identity and expression, marital status, national origin, political opinions or affiliations, genetic information and veteran status in all aspects of employment including recruitment, hiring, promotions, transfers, discipline, terminations, wage and salary administration, benefits, and training.
This is a time limited position.
Application must be submitted by 11:55 p.m. (ET) of the posting end date.
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