The department of Pathology is seeking applicants to fill the position of Biological Scientist III. The position will work as part of a team on NIH funded projects including the neuroanatomy of pancreatic innervation and islet transcriptomes (RNA studies) The incumbent will provide laboratory and data analysis services
This position's duties include, but are not limited to, handling human blood/tissue/cell/DNA/RNA samples to conduct research; data entry in databases (RedCap, Microsoft Access); attending internal and external laboratory meetings; preparation of data for presentations; writing methodologies for standard operating procedures and manuscript submission; working with immunolocalization including multifluorescence, in situ hybridization; fluorescence in situ hybridization; light microscopy; fluorescence, confocal microscopy and Lightsheet; digital imaging microscopy techniques.
Sample handling including animal and human cell culture; isolation of DNA or RNA and analysis by real-time/quantitative PCR or RNAsequencing; Immunohistochemistry/immunofluorescence; in situ hybridization; digital imaging and analysis including epifluorescence, confocal and LightSheet microscopy, and other laboratory bench work to support the above procedures.
Train staff and assistants in laboratory safety and other areas, as needed, to conduct their experiments. Maintenance of IACUC, EHS and IRB compliances. Data entry, data analysis by statistical programs and writing manuscripts, preparing presentations, and assisting with grant progress reports applications leading from lab studies.
Maintain the lab according to good laboratory practices. Evaluation of new equipment and maintain supplies and equipment required for the project.
Develop new assays for animal and human pancreas cell culture based on new, experimental conditions including microscopy with FACS, RNA, and protein analyses.
Other duties as assigned by PI.