Biological Scientist IV - Pediatrics Cellular Molecular Therapy

Apply now Job no: 522645
Work type: Staff Full-Time
Location: Main Campus (Gainesville, FL)
Categories: Health Profession, Other, Research/Scientific/Grants
Department:29092300 - MD-PEDS-CELL / MOLEC THERP DIV

Classification Title:

Biological Scientist IV - Pediatrics Cellular Molecular Therapy

Job Description:

The Department of Pediatrics, Cellular and Molecular, Dr. Zolotukhin Research Laboratory is accepting applications to fill the position of Biological Scientist IV. Dr. Zolotukhin’s research focuses on the directed evolution of combinatorial AAV capsid libraries, tissue-specific targeting of rAAV, and development of novel platforms for a large-scale rAAV manufacturing. The individual in this position is expected to function autonomously and troubleshoot complex scientific issues independently and design laboratory experiments.  Furthermore, this position will be responsible for the day-today research related activities to realize project timelines. Furthermore, this position will be responsible for the day-today research related activities to realize project timelines. Primary duties will include cell culture and molecular work, data collection and analysis, and laboratory management and compliance.

Responsibilities to include:

Research Activities: 

  • Develop and implement research activities to ensure adherence to the project/study timeline(s).
  • Oversee and monitor the implementation of research timeline(s). Provide regular updates to the Principal Investigator.
  • Cultivate/manage the expansion/use of cell lines following established laboratory methods.  Independently develop novel methods/techniques to troubleshoot errors.
  • Conduct molecular cloning experiments following established laboratory methods.  Independently develop methods/techniques to troubleshoot errors.
  • Complete plasmid purification related activities, primer design related activities, RNA and DNA isolation related activities, and perform real-time PCR related activities with moderate to minimal supervision.  Independently troubleshoot issues as they arise.
  • Prepare and maintain recombinant Adeno-associated virus stocks following established laboratory methods.  Independently develop methods/techniques to troubleshoot errors.
  • Coordinate and execute in vitro/in vivo studies exploring transfection of novel vectors
  • Search scientific literature to select and modify methods and procedures most appropriate for research goals
  • Adjust experimental designs as necessary based on experimental data analysis.
  • Maintain accurate laboratory records that record research methods, procedures, and results.

Laboratory Manager/Regulatory Compliance: 

  •  Responsible for managing daily operations of the laboratory.
  • Monitor and ensure regulatory compliance for all lab activities.
  • Order and reconcile of laboratory supplies in advance of planned experiment.
  • Coordinate and manage lab activities/schedule to achieve research objectives.  Notify the Principal Investigator of deviations from planned study timelines.
  • Monitoring and assisting other members to ensure research success and timelines.
  • Responsible for coordinating and scheduling personnel/volunteer lab activities to achieve research objective.
  • Maintaining, update and follow laboratory Standard Operation Procedures (SOPs) as needed to be in compliance with UF Environmental Health & Safety office.

Other Duties:

  •  Collaborate with the PI in completion of technical reports, research papers, scholarly articles, abstracts, or recommendations.
  • Collaborate with the PI in completion of figures for presentations, publications, or grants.
  • Collaborate with the PI in completion of grant proposals and progress reports to funding agencies.
  • General lab maintenance (washing dishes, cleaning benchtops, etc) of lab and equipment.
Expected Salary:

$60,000 - $65,000 annually; commensurate within this range based on qualifications and experience.

Minimum Requirements:

Bachelor’s degree in biology or a closely related field and thirteen years of relevant experience; Master’s degree in biology or a closely related field and eleven years of relevant experience; Doctoral degree in biology or a closely related field and eight years of relevant experience.

Preferred Qualifications:
  • Cell culture experience
  • rAAV packaging, purification, and tittering experience
  • Plasmid purification experience
  • AAV experience
Special Instructions to Applicants:
To be considered for this position, please upload your cover letter or letter of interest, resume, and a list of professional references.
 
Normal work schedule will be Monday through Friday, 8:00am to 5:00pm. Nights & weekends as needed for on-going research.

The University of Florida is committed to non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, gender identity and expression, marital status, national origin, political opinions or affiliations, genetic information and veteran status in all aspects of employment including recruitment, hiring, promotions, transfers, discipline, terminations, wage and salary administration, benefits, and training.

This is a time-limited position.
 
This requisition has been reposted. Previous applicants are still under consideration and need not apply

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

Health Assessment Required: Yes

 

Advertised: Eastern Daylight Time
Applications close: Eastern Daylight Time

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