This is a senior level Biological Scientist position in a research lab studying adeno-associated virus (AAV) gene therapy in skeletal and muscular diseases. The Biological Scientist is expected to be able to:
- Develop/Edit protocols to improve existing techniques or to incorporate new diagnostic procedures,
- Oversee their execution assigned experiments,
- Review, approve, or interpret scientific results,
- Maintain a necessary rodent colony to achieve research objectives,
- Conduct rodent procedures as necessary to complete research objectives,
- Present researching findings to PI and at laboratory meetings,
- Maintain laboratory safety programs and train personnel in laboratory safety techniques,
- Actively participate in the writing of grants, papers, and statements of work.
The Biological Scientist III must be able to perform the following essential functions:
Histological Work/Analysis: Collecting organs and other tissues and embedding the specimens for sectioning either with a microtome or cryotome (for frozen sections). Following the preparation of slides with sections tissues, staining with standard histology stains such as H&E, as well as with immunohistochemistry will be done. Conduct image capture and analysis of tissue following intervention studies.
Molecular Techniques and Analysis: Molecular cloning, Plasmid purification, Primer design, RNA and DNA isolation, Isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines; immunoassays to gene therapy vectors and transgenes, including ELISAs. This may include expansion/maintenance of cell lines and analysis of transfection/infection. Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots.
Animal Studies: Handling mice for setting up timed matings, weaning animals and colony maintenance. Participating in treating animals with pharmacological agents or AAV vectors either through i.p., i.m., or i.v. injection. Other duties with the mice may include doing echocardiography, sensory and motor function, force mechanics, and metabolic studies. Play a key role in regulatory management (IACUCs).
Biodistribution and Toxicology Studies: This includes working with the University of Florida IACUC, writing and reviewing study protocols, responding to Quality Assurance unit procedure and study audits and deviations, and documentation and evaluation of data for generating formal study reports. This function also requires coordination with any other contracted research organization necessary to perform the study, including pathologists and serum chemists. The hands on animal portion of this position includes performing small animal surgeries, daily health assessments, blood collections and small and large animal necropsies. These activities require strict adherence to written SOPs.
Regulatory Compliance: Ensures the laboratory is compliant with governing rules and regulations. Coordinate all IACUC and IRB (exempt) submissions/renewals. Ensures all Environmental Health and Safety (EH&S) standards are met. Train/ensure new laboratory members to ensure compliance standards are met.
Perform tasks and experiments as directed by the Principal Investigator.
Maintaining and updating laboratory Standard Operating Procedures (SOPs).
|Special Instructions to Applicants:
To be considered for this position, please upload your cover letter or letter of interest, resume, and a list of professional references.
Normal work schedule will be Monday through Friday, 8:00am to 5:00pm. Nights & weekends as needed for on-going research.
This is a time-limited position.
Application must be submitted by 11:55 p.m. (ET) of the posting end date.